Fabrication and functional characterization of engineered features on pyrolytic carbon
Advances in Manufacturing | Vol., Issue 4, 2016 |
pp.
Engineered features on pyrolytic carbon (PyC) have been reported to improve the functional performance of the bio-implants. This paper is focused on the functional characterization of micro-features created on the surface of PyC. Two different types of micro-features (wide channels and arrayed holes) have been created by micro-electrical discharge machining (micro-EDM). Two other micro-features (fine channels and micro-pillars) have been created by micromilling process. Coliform bacterial strain was isolated from a sample of water and grown on all four textured. Cell growth was carried out on an unmachined surface to see the behavior of the isolated bacterial strain on the textured/non-textured surfaces. The samples were examined under SEM before and after wash to see cell growth and cell adhesion capability of the textures. The wide channels by micro-EDM show the maximum cell growth but poor cell adhesion. 184% higher cell growth has been observed on the wide channels in comparison with unmachined surface. The fine channels by micro-milling show comparatively lower growth but the cell adhesion on this surface was found excellent. 71% cells remain unwashed after washing of the surface having fine channel textures. It means that the channels structure shows the maximum cell growth and adhesion independent of machining process.
DOI: https://doi.org/10.1007/s40436-016-0139-4 Cited: 0 Download: 0
