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Point-of-Care Testing for Abnormal Amino Acid Metabolism:Visualized Colorimetric Detection of Tryptophan Based on Au@MnO2Nanoparticles AITranslate

Yi Huang; Xiaoting Zhuang; Ruyi Zhang; Qinghui Yu; Ziwei Kuang; Chubei Qiu; Zhaofan Luo; Mingcong Rong; Changqing Yi
Guangzhou Key Laboratory of Sensing Materials and Devices/Center for Advanced Analytical Science/School of Chemistry and Chemical Engineering;Guangzhou Key Laboratory of Sensing Materials and Devices/Center for Advanced Analytical Science/School of Chemistry and Chemical Engineering;Guangzhou Key Laboratory of Sensing Materials and Devices/Center for Advanced Analytical Science/School of Chemistry and Chemical Engineering;Guangzhou Key Laboratory of Sensing Materials and Devices/Center for Advanced Analytical Science/School of Chemistry and Chemical Engineering;Guangzhou Key Laboratory of Sensing Materials and Devices/Center for Advanced Analytical Science/School of Chemistry and Chemical Engineering;Guangzhou Key Laboratory of Sensing Materials and Devices/Center for Advanced Analytical Science/School of Chemistry and Chemical Engineering;Guangzhou Key Laboratory of Sensing Materials and Devices/Center for Advanced Analytical Science/School of Chemistry and Chemical Engineering;Guangzhou Key Laboratory of Sensing Materials and Devices/Center for Advanced Analytical Science/School of Chemistry and Chemical Engineering;Guangzhou Key Laboratory of Sensing Materials and Devices/Center for Advanced Analytical Science/School of Chemistry and Chemical Engineering
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Publisher: Youke Publish Co., Ltd.
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Abstract AITranslate

In this study,a novel colorimetric detection method for tryptophan is developed using Au@MnO2NPs based on their redox reaction.Tryptophan etches the outer MnO2NPs shell and forms a stable protective layer outside the released AuNPs core in situ,accompanied by a noticeable color change from brown to pink.According to the absorbance ratio of 545 nm and 580 nm(A545/A580),a rapid(within 1 min),accurate,and specific detection method for tryptophan is constructed amidst other common amino acids.Coupling with a smartphone application,integrated Au@MnO2NPs-based portable test strips can be used for the point-of-care testing(POCT)of tryptophan.

KeyWords AITranslate

stable protective layer point care testing au mno nanoparticles detection method aunps core tryptophan detection colorimetric method colorimetric detection method
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DOI：

Chinese Library Classification Number:O657.3[理学];R446.1[理学—分析化学]

Citation Information:

In this study,a novel colorimetric detection method for tryptophan is developed using Au@MnO2NPs based on their redox reaction.Tryptophan etches the outer MnO2NPs shell and forms a stable protective layer outside the released AuNPs core in situ,accompanied by a noticeable color change from brown to pink.According to the absorbance ratio of 545 nm and 580 nm(A545/A580),a rapid(within 1 min),accurate,and specific detection method for tryptophan is constructed amidst other common amino acids.Coupling with a smartphone application,integrated Au@MnO2NPs-based portable test strips can be used for the point-of-care testing(POCT)of tryptophan.

quote

GB/T 7714-2015 [1] Yi Huang, Xiaoting Zhuang, Ruyi Zhang, et al. Journal of Analysis and Testing, 2025(4): 694-700.
MLA [1] Yi Huang, et al., Journal of Analysis and Testing, no. 4, 2025, pp. 694-700.
APA [1] Yi Huang, Xiaoting Zhuang, Ruyi Zhang, Qinghui Yu, Ziwei Kuang, Chubei Qiu, Zhaofan Luo, Mingcong Rong, & Changqing Yi. (2025). Journal of Analysis and Testing(4), 694-700.
IEEE [1] Yi Huang, Xiaoting Zhuang, Ruyi Zhang, Qinghui Yu, Ziwei Kuang, Chubei Qiu, Zhaofan Luo, Mingcong Rong, and Changqing Yi, Journal of Analysis and Testing, no. 4, pp. 694-700, 2025.