Dual-Signal Homogeneous Electrochemical System for Turn-On Assay of MicroRNA Based on Target-Switched Cyclic Amplification AITranslate

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Chinese Library Classification Number:O657.1[理学];R318[理学—分析化学]

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Dual-signal electrochemical sensors have inherent self-correction function to overcome disturbances of experimental and environmental factors,but suffer from electrodes'modification or functional materials'preparation,resulting in cumbersome operation,weak stability and poor recognition efficiency.Herein,we propose a dual-signal electrochemical strategy for microRNA senstive detection based on target-driven T7 exonuclease(T7 Exo)-mediated signal amplification that eliminates the above-mentioned drawbacks.The recognition of methylene(MB)and ferrocene(Fc)co-tagged ssDNA(MB/Fc-ssDNA)by miRNA-155 resulted in the formation of double-stranded nucleic acids,which switched T7 Exo-assisted catalytic digestion on MB/Fc-ssDNA,achieving 1:N amplified generation of MB-mononucleotides(mNs)and Fc-mNs.Compared with MB/Fc-ssDNA,MB-mNs and Fc-mNs exhibited significantly declined electrostatic repulsion force toward working electrode,and then simultaneously generated two signals at distinct potentials,in which dual-signal analysis of miRNA-155 was achieved with limit of detection down to~fM level.This work renders a novel thinking concept to develop high-performance electrochemical sensors for early and reliable diagnosis of miRNA-related diseases,advancing the rapid development of intelligent medicines.