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Immobilizing Enzyme Biocatalysts onto Porous Polymer Monoliths Prepared from Cocontinuous Polymer Blends AITranslate

Benoît Liberelle; Ebrahim Jalali Dil; Faezeh Sabri; Basil D. Favis; Gregory De Crescenzo; Nick Virgilio
Department of Chemical Engineering, Polytechnique Montreal;Research Center for High Performance Polymer and Composite Systems (CREPEC), Department of Chemical Engineering;Research Center for High Performance Polymer and Composite Systems (CREPEC), Department of Chemical Engineering;Research Center for High Performance Polymer and Composite Systems (CREPEC), Department of Chemical Engineering;Department of Chemical Engineering, Polytechnique Montreal;Research Center for High Performance Polymer and Composite Systems (CREPEC), Department of Chemical Engineering
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Publisher: ACS
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Abstract AITranslate

We demonstrate how macroporous poly(ε-caprolactone) monoliths, prepared from cocontinuous polymer blends, can be used to immobilize enzyme biocatalysts─herein demonstrated with glucose oxidase (GOx) and peroxidase (POx). The pores are covered with a self-assembled layer of aminated sub-μm silica particles, over which a thin, low-fouling carboxymethylated dextran (CMD) layer is grafted. GOx or POx is finally grafted onto the CMD layer. Our materials display a combination of strong enzyme immobilization, low nonspecific adsorption, and significant enzymatic activity. This approach constitutes a versatile technological platform for the immobilization of biomolecules onto polymer-based monoliths with precisely controlled porosity parameters for both batch and continuous-flow bioprocesses.

KeyWords AITranslate

cocontinuous polymer blend enzyme porosity surface modification biocatalysis monolith nanoparticles
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Basic Information:

DOI：https://doi.org/10.1021/acsapm.1c01144

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Citation Information:

We demonstrate how macroporous poly(ε-caprolactone) monoliths, prepared from cocontinuous polymer blends, can be used to immobilize enzyme biocatalysts─herein demonstrated with glucose oxidase (GOx) and peroxidase (POx). The pores are covered with a self-assembled layer of aminated sub-μm silica particles, over which a thin, low-fouling carboxymethylated dextran (CMD) layer is grafted. GOx or POx is finally grafted onto the CMD layer. Our materials display a combination of strong enzyme immobilization, low nonspecific adsorption, and significant enzymatic activity. This approach constitutes a versatile technological platform for the immobilization of biomolecules onto polymer-based monoliths with precisely controlled porosity parameters for both batch and continuous-flow bioprocesses.

quote

GB/T 7714-2015 [1] Benoît Liberelle, Ebrahim Jalali Dil, Faezeh Sabri, et al. ACS Applied Polymer Materials, 2021(3). DOI:10.1021/acsapm.1c01144.
MLA [1] Benoît Liberelle, et al., ACS Applied Polymer Materials, no. 3, 2021, https://doi.org/10.1021/acsapm.1c01144.
APA [1] Benoît Liberelle, Ebrahim Jalali Dil, Faezeh Sabri, Basil D. Favis, Gregory De Crescenzo, & Nick Virgilio. (2021). ACS Applied Polymer Materials(3). https://doi.org/10.1021/acsapm.1c01144
IEEE [1] Benoît Liberelle, Ebrahim Jalali Dil, Faezeh Sabri, Basil D. Favis, Gregory De Crescenzo, and Nick Virgilio, ACS Applied Polymer Materials, no. 3, 2021, doi: 10.1021/acsapm.1c01144. keywords: {cocontinuous polymer blend;enzyme;porosity;surface modification;biocatalysis;monolith;nanoparticles}