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Injectable biomimetic shellfish macromolecule conductive microcarriers loaded with adipose-derived stem cells for nerve repair in vivo AITranslate

Mengjiao Ma; Guodong Liu; Haoye Meng; Yudong Zheng; Jiang Peng; Yajie Xie; Yansen Wang; Wei He; Shuai Wei;
University of Science and Technology Beijing; University of Science and Technology Beijing; University of Science and Technology Beijing; University of Science and Technology Beijing; PLA Institute of Orthopedics; University of Science and Technology Beijing; University of Science and Technology Beijing; University of Science and Technology Beijing; PLA Institute of Orthopedics
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Publisher: Elsevier
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Abstract AITranslate

In regenerating and repairing the nerve injured by the congenital malformation or trauma, the conundrum of electrical signal transmission between the nerve cells in damaged nerve tissue remains an uncrossable barrier, owing to potential limitations of the tissue in repair. Filling conductive guiding cues into nerve conduits has been effective in stimulating functional nerve recovery. The present study presents a novel porous conductive chitosan/polydopamine (CS/PDA) microcarriers for nerve defect repair were prepared by the self-polymerization properties of mussel-like macromolecule dopamine. The porosity is more than 90%, and the electrical conductivity reaches 7 × 10−4 S/cm. The electrical signal was simulated by electrical stimulation in vitro. Compared with non-conductive microcarriers, conductive ones can effectively promote cell adhesion and proliferation. The conductive microcarriers loaded rat adipose-derived stem cells (rADSCs) were injected into nerve conduits and then transplanted into SD rats, with the autologous and the hollow groups as controls. The results of histology, immunohistochemical staining and gait analysis showed that CS/PDA had a promising ability to repair nerve defects in vivo. Graphical abstract The present study demonstrates novel porous conductive CS/PDA microcarriers were prepared by self-polymerization properties of mussel-like macromolecule dopamine to improve the microenvironment in nerve conduit for nerve defect repair. The electrical signal in vivo was simulated by electrical stimulation in vitro, compared with non-conductive microcarriers, conductive ones can effectively promote cell adhesion and proliferation. The conductive microcarriers loaded with rADSCs were injected into nerve conduits, and then transplanted into SD rats, with the autologous and the hollow groups as controls. The results of histology, immunohistochemical staining, and gait analysis verified the excellent capability of CS/PDA in repairing the nerve defects in vivo. Download : Download high-res image (191KB) Download : Download full-size image

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DOI：https://doi.org/10.1016/j.apmt.2021.101195

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Citation Information:

In regenerating and repairing the nerve injured by the congenital malformation or trauma, the conundrum of electrical signal transmission between the nerve cells in damaged nerve tissue remains an uncrossable barrier, owing to potential limitations of the tissue in repair. Filling conductive guiding cues into nerve conduits has been effective in stimulating functional nerve recovery. The present study presents a novel porous conductive chitosan/polydopamine (CS/PDA) microcarriers for nerve defect repair were prepared by the self-polymerization properties of mussel-like macromolecule dopamine. The porosity is more than 90%, and the electrical conductivity reaches 7 × 10−4 S/cm. The electrical signal was simulated by electrical stimulation in vitro. Compared with non-conductive microcarriers, conductive ones can effectively promote cell adhesion and proliferation. The conductive microcarriers loaded rat adipose-derived stem cells (rADSCs) were injected into nerve conduits and then transplanted into SD rats, with the autologous and the hollow groups as controls. The results of histology, immunohistochemical staining and gait analysis showed that CS/PDA had a promising ability to repair nerve defects in vivo. Graphical abstract The present study demonstrates novel porous conductive CS/PDA microcarriers were prepared by self-polymerization properties of mussel-like macromolecule dopamine to improve the microenvironment in nerve conduit for nerve defect repair. The electrical signal in vivo was simulated by electrical stimulation in vitro, compared with non-conductive microcarriers, conductive ones can effectively promote cell adhesion and proliferation. The conductive microcarriers loaded with rADSCs were injected into nerve conduits, and then transplanted into SD rats, with the autologous and the hollow groups as controls. The results of histology, immunohistochemical staining, and gait analysis verified the excellent capability of CS/PDA in repairing the nerve defects in vivo. Download : Download high-res image (191KB) Download : Download full-size image

quote

GB/T 7714-2015 [1] Mengjiao Ma, Guodong Liu, Haoye Meng, et al. Applied Materials Today, 2021(25). DOI:10.1016/j.apmt.2021.101195.
MLA [1] Mengjiao Ma, et al., Applied Materials Today, no. 25, 2021, https://doi.org/10.1016/j.apmt.2021.101195.
APA [1] Mengjiao Ma, Guodong Liu, Haoye Meng, Yudong Zheng, Jiang Peng, Yajie Xie, Yansen Wang, Wei He, & Shuai Wei. (2021). Applied Materials Today(25). https://doi.org/10.1016/j.apmt.2021.101195
IEEE [1] Mengjiao Ma, Guodong Liu, Haoye Meng, Yudong Zheng, Jiang Peng, Yajie Xie, Yansen Wang, Wei He, and Shuai Wei, Applied Materials Today, no. 25, 2021, doi: 10.1016/j.apmt.2021.101195.