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Study on bioactive PEGDA/ECM hybrid bi-layered hydrogel scaffolds fabricated by electro-writing for cartilage regeneration AITranslate

Yu Han; Meifei Lian; Chenyu Zhang; Bo Jia; Qiang Wu; Benlin Sun; Zhiguang Qiao; Binbin Sun; Kerong Dai
The First Affiliated Hospital of Zhengzhou University; Shanghai Jiao Tong University School of Medicine; Shanghai Jiao Tong University School of Medicine; Shanghai Jiao Tong University School of Medicine; Shanghai Jiao Tong University School of Medicine; Shanghai Jiao Tong University School of Medicine; Shanghai Jiao Tong University School of Medicine; Shanghai Jiao Tong University School of Medicine; Shanghai Jiao Tong University School of Medicine
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Publisher: Elsevier
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Abstract AITranslate

Highlights • This study aimed to investigate the effectivity of a polyethylene glycol diacrylate (PEGDA) and extracellular matrix (ECM) hydrogel to mix BMSCs with TGF-β1, BMP-7, and IGF-1 through a high-precision electro-writing printing (EWP) printing method to produce a high-precision, high-porosity, hierarchical cartilage repair scaffold with high biocompatibility and biological activity. • Through in vitro and in vivo experiments, the biological activity, anti-inflammatory activity, and cartilage repair effect of the scaffold were verified. Articular cartilage injury is a common disease in orthopedics. Because of the poor repair ability of articular cartilage itself, medical intervention is often required for articular cartilage injury. A promising tool for cartilage injury repair is electro-writing (EW), a 3D printing technology, which can be used to prepare high-precision and high-porosity layered active scaffolds, thereby allowing it to imitate the layered structure of cartilage. In this study, we developed a hybrid ink with good EW printability and formability based on polyethylene glycol diacrylate (PEGDA) and extracellular matrix, in which cells can be mixed for printing. Moreover, growth factors were also integrated into the printed material, such that the upper layer of the scaffold was loaded with transforming growth factor-β1 (TGF-β) + bone morphogenetic protein-7 (BMP-7) and the lower layer was loaded with TGF-β+insulin growth factor-1 (IGF-1). The whole scaffold encompassed layered active ingredients and can functionally repair damaged cartilage after being implanted in the body. In summary, this study explored the printability and formability of EW hydrogel inks, based on which an original hybrid cell hydrogel ink was developed for constructing a bioactive cartilage repair scaffold capable of inducing cell differentiation to promote cartilage repair. Graphic Download : Download high-res image (200KB) Download : Download full-size image

KeyWords AITranslate

Hybrid hydrogel bilayer scaffolds Electrowriting Cartilage injury Bone marrow mesenchymal stem cells AGC aggrecan aggrecan BC blank control blank control BMP7 bone morphogenetic protein7 bone morphogenetic protein7 BMSC bone marrow mesenchymal stem cell bone marrow mesenchymal stem cell BSA bovine serum albumin bovine serum albumin COL II collagen II collagen II CS composite scaffold composite scaffold DAPI 4′ 6diamidino2phenylindole 4′ 6diamidino2phenylindole ECM extracellular matrix extracellular matrix ELISA enzymelinked immunosorbesnt assay enzymelinked immunosorbesnt assay EW electrowriting electrowriting GelMA gelatin methacryloyl gelatin methacryloyl HAMA methacrylated hyaluronic acid methacrylated hyaluronic acid HE hematoxylin & eosin hematoxylin & eosin IFNγ interferonγ interferonγ IGF1 insulinlike growth factor1 insulinlike growth factor1 IL1 interleukin1 interleukin1 iNOS inducible nitric oxide synthase inducible nitric oxide synthase PBS phosphate buffered saline phosphate buffered saline PCL poly(εcaprolactone) poly(εcaprolactone) PEG polyethylene glycol polyethylene glycol PEGDA polyethylene glycol diacrylate polyethylene glycol diacrylate PEO poly(ethylene oxide) poly(ethylene oxide) PRG4 proteoglycan4 proteoglycan4 RTPCR reverse transcriptionpolymerase chain reaction reverse transcriptionpolymerase chain reaction SEM scanning electron microscopy scanning electron microscopy SS simple scaffold simple scaffold TGFβ1 transforming growth factorβ1 transforming growth factorβ1 TNF tumor necrosis factor tumor necrosis factor
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Basic Information:

DOI：https://doi.org/10.1016/j.apmt.2022.101547

Chinese Library Classification Number:

Citation Information:

Highlights • This study aimed to investigate the effectivity of a polyethylene glycol diacrylate (PEGDA) and extracellular matrix (ECM) hydrogel to mix BMSCs with TGF-β1, BMP-7, and IGF-1 through a high-precision electro-writing printing (EWP) printing method to produce a high-precision, high-porosity, hierarchical cartilage repair scaffold with high biocompatibility and biological activity. • Through in vitro and in vivo experiments, the biological activity, anti-inflammatory activity, and cartilage repair effect of the scaffold were verified. Articular cartilage injury is a common disease in orthopedics. Because of the poor repair ability of articular cartilage itself, medical intervention is often required for articular cartilage injury. A promising tool for cartilage injury repair is electro-writing (EW), a 3D printing technology, which can be used to prepare high-precision and high-porosity layered active scaffolds, thereby allowing it to imitate the layered structure of cartilage. In this study, we developed a hybrid ink with good EW printability and formability based on polyethylene glycol diacrylate (PEGDA) and extracellular matrix, in which cells can be mixed for printing. Moreover, growth factors were also integrated into the printed material, such that the upper layer of the scaffold was loaded with transforming growth factor-β1 (TGF-β) + bone morphogenetic protein-7 (BMP-7) and the lower layer was loaded with TGF-β+insulin growth factor-1 (IGF-1). The whole scaffold encompassed layered active ingredients and can functionally repair damaged cartilage after being implanted in the body. In summary, this study explored the printability and formability of EW hydrogel inks, based on which an original hybrid cell hydrogel ink was developed for constructing a bioactive cartilage repair scaffold capable of inducing cell differentiation to promote cartilage repair. Graphic Download : Download high-res image (200KB) Download : Download full-size image

quote

GB/T 7714-2015 [1] Yu Han, Meifei Lian, Chenyu Zhang, et al. Applied Materials Today, 2022(28). DOI:10.1016/j.apmt.2022.101547.
MLA [1] Yu Han, et al., Applied Materials Today, no. 28, 2022, https://doi.org/10.1016/j.apmt.2022.101547.
APA [1] Yu Han, Meifei Lian, Chenyu Zhang, Bo Jia, Qiang Wu, Benlin Sun, Zhiguang Qiao, Binbin Sun, & Kerong Dai. (2022). Applied Materials Today(28). https://doi.org/10.1016/j.apmt.2022.101547
IEEE [1] Yu Han, Meifei Lian, Chenyu Zhang, Bo Jia, Qiang Wu, Benlin Sun, Zhiguang Qiao, Binbin Sun, and Kerong Dai, Applied Materials Today, no. 28, 2022, doi: 10.1016/j.apmt.2022.101547. keywords: {Hybrid hydrogel bilayer scaffolds;Electrowriting;Cartilage injury;Bone marrow mesenchymal stem cells;AGC aggrecan;aggrecan;BC blank control;blank control;BMP7 bone morphogenetic protein7;bone morphogenetic protein7;BMSC bone marrow mesenchymal stem cell;bone marrow mesenchymal stem cell;BSA bovine serum albumin;bovine serum albumin;COL II collagen II;collagen II;CS composite scaffold;composite scaffold;DAPI 4′;6diamidino2phenylindole;4′;6diamidino2phenylindole;ECM extracellular matrix;extracellular matrix;ELISA enzymelinked immunosorbesnt assay;enzymelinked immunosorbesnt assay;EW electrowriting;electrowriting;GelMA gelatin methacryloyl;gelatin methacryloyl;HAMA methacrylated hyaluronic acid;methacrylated hyaluronic acid;HE hematoxylin & eosin;hematoxylin & eosin;IFNγ interferonγ;interferonγ;IGF1 insulinlike growth factor1;insulinlike growth factor1;IL1 interleukin1;interleukin1;iNOS inducible nitric oxide synthase;inducible nitric oxide synthase;PBS phosphate buffered saline;phosphate buffered saline;PCL poly(εcaprolactone);poly(εcaprolactone);PEG polyethylene glycol;polyethylene glycol;PEGDA polyethylene glycol diacrylate;polyethylene glycol diacrylate;PEO poly(ethylene oxide);poly(ethylene oxide);PRG4 proteoglycan4;proteoglycan4;RTPCR reverse transcriptionpolymerase chain reaction;reverse transcriptionpolymerase chain reaction;SEM scanning electron microscopy;scanning electron microscopy;SS simple scaffold;simple scaffold;TGFβ1 transforming growth factorβ1;transforming growth factorβ1;TNF tumor necrosis factor;tumor necrosis factor}