Self-Enhanced Electrochemiluminescence Microreactors Based on Bimetallic Porphyrin Covalent Organic Frameworks for MicroRNA Detection AITranslate

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Chinese Library Classification Number:O657.1[理学]

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Controlling the intermolecular charge transfer between co-reactants and emitters represents a promising strategy for elucidating the luminescent mechanism and enhancing the efficiency of electrochemiluminescence(ECL)systems.Herein,a self-enhanced ECL microreactor was prepared by bimetallic Zn-/Co-porphyrin covalent organic framework(COF_Zn/CoP-Ph@MWCNTs),in which co-reaction accelerators(cobalt porphyrin)and luminophores(zinc porphyrin)were precisely woven into the COFs skeleton via covalent bonds.Owing to the unique topological structure,the rational arrangement of coreaction accelerators and luminophores in a spatially confined environment effectively controlled the intermolecular charge transfer.And the spatially restricted environment formed by the COF_Zn/CoP-Ph@MWCNTs microreactor avoided the problem of disordered diffusion of active intermediates,leading to an enhanced ECL response.Thanks to the synergistic impacts of the factors above,the ECL intensity of the bimetallic COF_Zn/CoP-Ph@MWCNTs was 10.2-folds stronger than the zinc porphyrin monomer.Therefore,the self-enhanced ECL sensor was constructed for miRNA-155 detection via an“on-off”ECL quenching mechanism and a cycling amplification strategy,displayed a broad linear range(0.10 pM–10μM)and a low limit of detection(0.51 fM).In brief,studying the programmable structures and tunable electronic paths of high-performance COF materials would provide good access for the engineering self-enhanced ECL system and the construction of ultrasensitive sensors.