Magnetic Force Microscopy and Nanoindentation on 3D Printed Magnetic Scaffolds for Neuronal Cell Growth AITranslate

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DOI：https://doi.org/10.1021/acsapm.3c02565

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This study investigated the physicochemical properties of 3D printed sodium alginate (SA)/poly(vinyl alcohol) (PVA)-magnetic nanoparticle (MNP) hydrogels that were subsequently cross-linked using Ca2+ ions via postspraying. The rheological properties of the precursor hydrogels were assessed because they play a crucial role in printability. The SA/PVA composition of 12/8 wt %, both in the absence and presence of MNPs at concentrations of 1.0 mg/mL, 2.5 mg/mL, or 5.0 mg/mL, displayed good printability. Magnetic force microscopy (MFM) evidenced the random distribution of MNPs on the hydrogel surface and the aggregation of magnetic clusters with increasing MNP content. Nanoindentation tests using a silica colloidal probe allowed estimating the elastic modulus values of swollen 3D printed scaffolds. These values ranged from 1.0 MPa (SA12/PVA8) to 7.2 MPa (SA/PVA-MNP5). Confocal microscopy confirmed the presence of cells within the interior of the 3D printed scaffolds. The cytocompatibility or cytotoxicity assays showed that all 3D printed scaffolds were cytocompatible with HT-22 cells.