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DNA Origami Nanostructures with Scaffolds Obtained from Rolling Circle Amplification AITranslate

Mengyuan Xu; Chi Zhang; Cunjun Zhang; Yiling Zhao; Zhi Qi; Chunhai Fan; Jie Chao; Bryan Wei;
Tsinghua University; Tsinghua University; Tsinghua University; Peking University; Peking University; Shanghai Jiao Tong University; Nanjing University of Posts and Telecommunications; Tsinghua University
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Publisher: ACS
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Abstract AITranslate

As a guiding backbone of origami structural folding, the long single-stranded scaffold is the key enabler of all kinds of complex DNA nanostructures. However, the folding of most origami nanostructures depends heavily on very limited species of scaffolds. It is desirable to have a wider choice of scaffolds for the extended design space. Here we develop a new method based on rolling circle amplification to prepare scaffolds of custom lengths and sequences. We demonstrate that amplicons templated by viral genome and plasmid can serve as scaffolds to fold DNA origami nanostructures.

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DOI：https://doi.org/10.1021/acsmaterialslett.9b00484

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Citation Information:

As a guiding backbone of origami structural folding, the long single-stranded scaffold is the key enabler of all kinds of complex DNA nanostructures. However, the folding of most origami nanostructures depends heavily on very limited species of scaffolds. It is desirable to have a wider choice of scaffolds for the extended design space. Here we develop a new method based on rolling circle amplification to prepare scaffolds of custom lengths and sequences. We demonstrate that amplicons templated by viral genome and plasmid can serve as scaffolds to fold DNA origami nanostructures.

quote

GB/T 7714-2015 [1] Mengyuan Xu, Chi Zhang, Cunjun Zhang, et al. ACS Materials Letters, 2020(2). DOI:10.1021/acsmaterialslett.9b00484.
MLA [1] Mengyuan Xu, et al., ACS Materials Letters, no. 2, 2020, https://doi.org/10.1021/acsmaterialslett.9b00484.
APA [1] Mengyuan Xu, Chi Zhang, Cunjun Zhang, Yiling Zhao, Zhi Qi, Chunhai Fan, Jie Chao, & Bryan Wei. (2020). ACS Materials Letters(2). https://doi.org/10.1021/acsmaterialslett.9b00484
IEEE [1] Mengyuan Xu, Chi Zhang, Cunjun Zhang, Yiling Zhao, Zhi Qi, Chunhai Fan, Jie Chao, and Bryan Wei, ACS Materials Letters, no. 2, 2020, doi: 10.1021/acsmaterialslett.9b00484.